ABSTRACT
Objective: To validate intra- and inter-class correlation coefficients of a transparent 3D-TC protocol and investigate relationships between different axial rotations. Methods: Twenty unilateral knee TCs (iSite - Philips) were evaluated by means of a transparent 3D-TC OsiriX Imaging Software (v.3.9.4), 3D MPR protocol. Mathematical model of femoral tunnel projections acquired on vertical and horizontal rotations from -20 to +20 degrees. Height (h'/H) and length (t'/T) of tunnel projections have been analyzed by the Bernard and Hertel's method. Statistics: power of study=80%, ICC, ANOVA, p<0.05 (SPSS-19). Results: Transparent 3D-TC showed high reliability of both intra-observer (h'/H=0.941; t'/T=0.928, p<0.001) and inter-observer (h'/H=0.921; t'/T=0.890, p<0.001) ICC. ACL Length (t'/T) and Height (h'/H) projections were statistically different on vertical and horizontal rotations: p=0.01 and p<0.001, respectively. Conclusion: This new transparent 3D-TC protocol is an accurate and reproducible method that can be applied for ACL femoral tunnel or footprint measurement with high ICC reliability. Level of Evidence II, Descriptive Laboratory Study.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Image Processing, Computer-Assisted , Image Interpretation, Computer-Assisted , Tomography, X-Ray Computed , Anterior Cruciate Ligament/anatomy & histology , KneeABSTRACT
The aim of this work was to perform the histological characterization of the C. sativa leaves of three Portuguese cultivars to establish comparison among the leaves of the different quadrants in accord and with the cardinal points of the tree top and among different cultivars of this species, using light microscopy (OM) and scanning electron microscopy (SEM). Measurements were also carried out for the leaf tissue thickness, stomatal density, leaf area in the four tree top quadrants. The leaves turned to the North had lesser thickness of mesophyll mainly due to lower amount of palisade parenchyma. The stomatal density was significantly lower in these leaves, unlike the leaf area that has the highest expression.